| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073195 |
| Chromosome: | chromosome 1 |
| Location: | 4215994 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028450 | (1 of 1) PTHR30544//PTHR30544:SF2 - 23S RRNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGATCCTGTGACGAGGGGCTGGGTGCACGAGGTGGCATCACAAGCAT |
| Internal bar code: | GTATTTTCTGGGACGGGCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 64.4068 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGGCAGGGAGGGGTGAT |
| Suggested primer 2: | CCTGTGTCTAGGGAGGAGCT |