Insertion junction: CLIP2.073204_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073204
Chromosome:chromosome 13
Location:4800120
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g604700 PGP2 (1 of 1) K08744 - cardiolipin synthase (CRLS); Phosphatidylglycerolphosphate synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCCTATACTGCGCCACTGTTGAAATTGAAGGGACCCCTCGTCCACTG
Internal bar code:CTATATCTGTGGGCGCTGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1792
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCGTGTCACTGGTGTCAT
Suggested primer 2:ATACATGCGGCATCGTAGCA