| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073205 |
| Chromosome: | chromosome 12 |
| Location: | 9277574 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542550 | (1 of 13) 2.3.1.225 - Protein S-acyltransferase / S-protein acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGAAGCAAGTATTGCAGCACCTGATTATCCGTTTGTTGAATTGTGCAG |
| Internal bar code: | TATTTCTCAAGGTCCATAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTCTGGACTCTGCTGCT |
| Suggested primer 2: | GTATGGAGGTGGTGGTGTGG |