| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073209 |
| Chromosome: | chromosome 4 |
| Location: | 2486456 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222000 | (1 of 2) PTHR22847//PTHR22847:SF459 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTTGGAAGAGGGATCGGTGGCATATGTGCCAAGCATGCATGGGACAG |
| Internal bar code: | GGATTTTGCAAATCTGTTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2040 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGCGGCACATGAGTTGT |
| Suggested primer 2: | CCACTTTGTGAACAGCGTGG |