| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073223 |
| Chromosome: | chromosome 5 |
| Location: | 507215 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244236 | (1 of 1) K07442 - tRNA (adenine57-N1/adenine58-N1)-methyltransferase [EC:2.1.1.219 2.1.1.220] (TRM61, GCD14) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACAGCGCCTCGCTGGTGCGCTGGACCTGTGCGCGTGGGAGAGGCCGG |
| Internal bar code: | CATCGATGCGCCTCGTGTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 31.5789 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCAGCAATGTACAAGGT |
| Suggested primer 2: | GGAAGCCGATGAGCTAGTCC |