Insertion junction: CLIP2.073252_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.073252
Chromosome:chromosome 10
Location:5817737
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g460350 PGA4 Putative phospholipid/glycerol acyltransferase; (1 of 3) 2.3.1.25 - Plasmalogen synthase 3'UTR
Cre10.g460400 (1 of 1) PF00642//PF04564 - Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH) // U-box domain (U-box) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGAGAGACATGAAGTCCTCGCCACCTTGTCCTCGTCTGTCCTCACCCCA
Internal bar code:CCACTCCAGAGACAGGCTCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3714
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGGTGTGTCATCCTGGC
Suggested primer 2:CGGAGTTCGACCTGTTGACA