| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073254 |
| Chromosome: | chromosome 5 |
| Location: | 3667186 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800583 | (1 of 12) IPR011010//IPR013762 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCTGACCCAGCCGGCCCAGCTGACCCAGCCCGCCCCCTCCCCACCC |
| Internal bar code: | TCCTATTAAGACGGACACCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 71 |
| LEAP-Seq percent confirming: | 4.7619 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTTACGTGCAGCACTAG |
| Suggested primer 2: | AGCCCCTAGTGCTGTACGTA |