| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073267 |
| Chromosome: | mating type plus |
| Location: | 10215 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252250 | MTP0428 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAACCAGTGGGTTGCTTCTGCATATAATAATCTACCTCTACCACCATG |
| Internal bar code: | ACCGCTAGTCTAGAAGGGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2009 |
| LEAP-Seq percent confirming: | 21.2766 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAACCCCTCATGCAGCCA |
| Suggested primer 2: | GGTCAAGGTTGGTGGGTTCA |