| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073271 |
| Chromosome: | chromosome 17 |
| Location: | 2445376 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715000 | HSP33 | Heat shock protein 33; (1 of 1) K04083 - molecular chaperone Hsp33 (hslO) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTTGGTCTGCTCGCTGTCCACCTGGGGGAGGGGGCGACGGAGGTTGCC |
| Internal bar code: | TATGGTTATTTGCCAGCGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 406 |
| LEAP-Seq percent confirming: | 2.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCGAGCGAGGAGGAGAGT |
| Suggested primer 2: | CACCTCCTTCTTGCCCAGAG |