| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073273 |
| Chromosome: | chromosome 15 |
| Location: | 5588721 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre26.g756947 | (1 of 1) PF15253 - SCL-interrupting locus protein N-terminus (STIL_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGCGGGCAGGCAGGCAGAGGAGAGGAGAGGGGAGGGCAGAGGGGCAG |
| Internal bar code: | AGCCTGCCGGACGCGCCATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGATGAGGGAGGGGAAGG |
| Suggested primer 2: | ACAGCTTTGGAACCCACACA |