| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073290 |
| Chromosome: | chromosome 6 |
| Location: | 1992152 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265000 | HTR13 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTTGGGCATGATCGTCACACGCTTGGCGTGGATGGCGCACAGGTTGG |
| Internal bar code: | GTACGAAACAAAGTTCGATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3288 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATAGCGGGCTTGGTAAT |
| Suggested primer 2: | AGCCTGCTCAATCGTTCACA |