Insertion junction: CLIP2.073298_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.073298
Chromosome:chromosome 2
Location:5883126
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre02.g117400 (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED CDS
Cre02.g117410 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCACCTGAAACAAGTCCAGTGCCACCTCCTCCTTCTCGTCCTCCTCGT
Internal bar code:TTTCATTTGTCCTGTTGTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:620
LEAP-Seq percent confirming:76.6667
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCAACAAACTGCTTGGCA
Suggested primer 2:AGTCCCTACAGTCCCTGGTC