| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073302 |
| Chromosome: | chromosome 6 |
| Location: | 7167808 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298350 | FAP224 | Flagellar Associated Protein 224; (1 of 1) PTHR14208//PTHR14208:SF2 - BASIC LEUCINE ZIPPER AND W2 DOMAIN-CONTAINING PROTEIN // PROTEIN KRASAVIETZ | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACTATCGCTAGCCGGAAGTAGGGGCGGCTGACAGTCAGACTGTTTCG |
| Internal bar code: | GGCAGTCACTCGGCGTGTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1273 |
| LEAP-Seq percent confirming: | 91.2281 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCAAGTGCTTCACCGAT |
| Suggested primer 2: | AGAACTCCTCTGCAATGGCC |