| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073305 |
| Chromosome: | chromosome 12 |
| Location: | 8903622 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545850 | FAP363 | Flagellar Associated protein 363; (1 of 1) PTHR19375:SF152 - HEAT SHOCK 70 KDA PROTEIN COGNATE 1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTGGGCCGCTAAGGTGGTGCAGCCCCCGCTAGCCCTTCAGGTGCGT |
| Internal bar code: | TTGTTGAGTTGTCGAAATGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1073 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGGATTATTTGCTGTGCG |
| Suggested primer 2: | CCTGTTTCGGCCCCTAACAT |