| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073320 |
| Chromosome: | chromosome 9 |
| Location: | 208366 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386743 | (1 of 1) 2.1.1.305 - 8-demethyl-8-alpha-L-rhamnosyl tetracenomycin-C 2'-O-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCTTTGCTTAACATTGTTTGACTTTGCACATTTGAAACTGGCATCG |
| Internal bar code: | ATTGTCGAGCTGGTGGGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1820 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATATGTGCACGCGTGTTCC |
| Suggested primer 2: | GAACAACCTCATCGCCCAGA |