| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073323 |
| Chromosome: | chromosome 7 |
| Location: | 1256301 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321900 | (1 of 6) 3.1.26.11 - Ribonuclease Z / tRNase Z | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTTGCACGAGTATTTGAGAGAAACTACATCACTTGATTGAAAACATA |
| Internal bar code: | CAGTGTATTCGGTTTTCTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1337 |
| LEAP-Seq percent confirming: | 17.9104 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTGCCAGTGGGATGTCC |
| Suggested primer 2: | GGAAGGGAAGGGGCTGTAAC |