| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.073325 |
| Chromosome: | plastome |
| Location: | 102245 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802308 | ChreCp044,2716995,psbL | photosystem II reaction center subunit XII; (1 of 1) K02713 - photosystem II PsbL protein (psbL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAATGTTATCCCCTTCCCCTTTGGGTAAATAAATTTTAGTGGAAGGTAC |
| Internal bar code: | AGTGTTGAGTATCTGAAATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCAATCGTTACTGGAACT |
| Suggested primer 2: | GCGGCTGTGGCTGTAGATAT |