| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073326 |
| Chromosome: | chromosome 10 |
| Location: | 4493620 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGCGCGGGGGCACCGCGTGTCATGCAGGCCACAGGGACGCGCGAATA |
| Internal bar code: | CATCGAACTGGGTGCGTGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 16.2791 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTCCTGTGGAGCAGCAG |
| Suggested primer 2: | GGTCATCCTCCAAGCTGCTT |