| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073368 |
| Chromosome: | chromosome 2 |
| Location: | 3922232 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101950 | TRM2B,TMU2 | tRNA (uracil-5)-methyltransferase; (1 of 1) K15332 - tRNA (uracil-5-)-methyltransferase [EC:2.1.1.-] (TRMT2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGGTCGAGCGCGGCAAAGGCGGGAGAGTCCAGGGAGGTGGGCGCCGC |
| Internal bar code: | TCCCCCGTTTGGGCCTCCCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGAGGGAGGTATGGGAC |
| Suggested primer 2: | GGATGTAGGGGTGAGGAGGT |