| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073368 |
| Chromosome: | chromosome 8 |
| Location: | 1200644 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363250 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCCTAGGCAAGCCCAAGAGCCTGTGGCCAGATCGATGCCATATCCA |
| Internal bar code: | AGATGTAGATCTTTTTGTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3904 |
| LEAP-Seq percent confirming: | 75.3425 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATCATGGGTCATTGGC |
| Suggested primer 2: | ACAAGGCAAACACCTCCACA |