Insertion junction: CLIP2.073372_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.073372
Chromosome:chromosome 17
Location:4058303
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g728700 (1 of 1) K11228 - mitogen-activated protein kinase kinase kinase (STE11) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTCAGAAGCTAAATCGAAACAGCAGTGAAGCTTGGGTGTCCGACACTG
Internal bar code:AGGGCTTAGGGAGGACCAGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1414
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTAGGACGGCGCTTTTCA
Suggested primer 2:CGCCGCTGATTTTGATCTGG