| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073391 |
| Chromosome: | chromosome 14 |
| Location: | 4041435 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633350 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCCGCAATTGCAGCCGCAGAGCCTGTGGCTGCCACAGGCGCAGCAGC |
| Internal bar code: | ATCGGGTTACTGTAACATTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 10.7438 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 121 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCCTTCTTCGCCTTCTT |
| Suggested primer 2: | TTGAGGAGGGAAGAGGGGTT |