| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.073395 |
| Chromosome: | chromosome 17 |
| Location: | 4811362 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g733400 | (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGACGGTGGTGTCACTTCGCGTCCTTCAGCTTACGTGGGTTGATGG |
| Internal bar code: | GGAGCTGGGGGTCGATGGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCGACTTGGAGGTTGAG |
| Suggested primer 2: | AAGAGTCACGTTGCTCTCCG |