| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073396 |
| Chromosome: | chromosome 6 |
| Location: | 2565631 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269601 | (1 of 3) 2.4.1.19 - Cyclomaltodextrin glucanotransferase / Cyclodextrin glycosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGGCGGCAAAACAAACTTGCGCAATCGATCCAAGACGTCCTTCCCAG |
| Internal bar code: | TTAAACTCATCTACGCATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACTGTCAAACCGCACAC |
| Suggested primer 2: | TCAGCTCGTCCACTTGTGTC |