| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073412 |
| Chromosome: | chromosome 12 |
| Location: | 5528339 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530450 | (1 of 4) IPR001841//IPR018957 - Zinc finger, RING-type // Zinc finger, C3HC4 RING-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAATGTCTTTCGCCTTCCGAATGGCTTGACTAAATGCTAACATGGCA |
| Internal bar code: | AGGAGGTGATGCGGTAAGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4135 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGTTGGACCAGGAGCAG |
| Suggested primer 2: | TACTGCACTTCACCCCGTTC |