Insertion junction: CLIP2.073422_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.073422
Chromosome:chromosome 16
Location:5371893
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683350 TEH6 Acyl-CoA thioesterase-like protein; (1 of 2) K17361 - acyl-coenzyme A thioesterase 9 [EC:3.1.2.-] (ACOT9) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAATTTGGAAAAAGTTAATGATAATTCGCGTTCCAAATGAAAAAAACTTA
Internal bar code:TACGAACCATTATGTCGGTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3785
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACTCGCTCAAACTCAGT
Suggested primer 2:CAGTTTTGCGTTCGTGAGCA