Insertion junction: CLIP2.073430_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.073430
Chromosome:chromosome 10
Location:5018494
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g454600 FAP15 Flagellar Associated Protein 415 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCTAGGCGCTAAATGCGCACGAATTGACAGTTTAGGTCTGGCAAGAAG
Internal bar code:CAGCGCTACTTCCGTAATTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:274
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACTTCTCTGAAGGGCCC
Suggested primer 2:AGAATCGCGGGACAACAAGT