| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073435 |
| Chromosome: | chromosome 3 |
| Location: | 6759920 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196550 | Found in Chlamydomonas, Volvox and Micromonas but not strongly conserved elsewhere; (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGTACGTGTGGCCATGTGGTGGCGTTAGACTACCGTATGAGCTGGCA |
| Internal bar code: | AACCTCCAGTTGTCGAGACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5684 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 117 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCTCGCCAGTGAAGTCC |
| Suggested primer 2: | CTTATCCTGCGGCAGAGGAG |