| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073435 |
| Chromosome: | chromosome 3 |
| Location: | 6759929 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196550 | Found in Chlamydomonas, Volvox and Micromonas but not strongly conserved elsewhere; (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAAATGTACTTCAGCCCTATCTACCCCGCTCAGGCTCGCTCCTCTCTG |
| Internal bar code: | CGCTGAGGTCTTATTACACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2212 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTATCCTGCGGCAGAGGAG |
| Suggested primer 2: | TTTCCTCGCCAGTGAAGTCC |