| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073451 |
| Chromosome: | chromosome 7 |
| Location: | 5672898 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352750 | MRS3 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATGGGCAAGCGCAGGCGGCATCTTGCACGGCGAGCCCAGGCTGTAG |
| Internal bar code: | CTGAAGTCATTCTAGTCCCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCAGCTCATCATTCAGC |
| Suggested primer 2: | CATCGACCTCATCCTCACGG |