| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073459 |
| Chromosome: | chromosome 12 |
| Location: | 5484003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529950 | EIF4G1,EIF4G | (1 of 2) K03260 - translation initiation factor 4G (EIF4G); Eukaryotic translation initiation factor 4, gamma subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTCCCCTTAAAACGAGGCCTCCGGGCCCCACAATGGGGGCGCCTGC |
| Internal bar code: | TTGCTACTCGTGTTAGCTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4337 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCGAAGACGTTTAGGCA |
| Suggested primer 2: | AAGCTTTCCTCCCATCACGG |