| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073469 |
| Chromosome: | chromosome 6 |
| Location: | 8383450 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCCAGCGGCGGCGGCGACAGACCCAGCAGCGCTACAGGCATGTGC |
| Internal bar code: | CCGCTGATAGATGTGTCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAAGTGTCCGCCATCGCT |
| Suggested primer 2: | CTTCCTCATCCTCGCCGTAC |