Insertion junction: CLIP2.073472_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073472
Chromosome:chromosome 17
Location:1894714
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709750 (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGATGCCCCACGAAACCCCTTCACATGCCCGAACACCCCTCGCAACAAA
Internal bar code:GGGGAGAGGTAACGACGATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4392
LEAP-Seq percent confirming:52.9412
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCAGCTTCATGTCTGC
Suggested primer 2:CTGCCTGCACATGATCATGC