Insertion junction: CLIP2.073489_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073489
Chromosome:chromosome 7
Location:5954406
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g354500 HSF2 (1 of 2) K09419 - heat shock transcription factor, other eukaryote (HSFF); Heat shock transcription factor 2 outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCTGACTACTTGTGGTGCGCGTGCCGCGATCTTGGTGGTGCCTGTGC
Internal bar code:TTTGGGATTTATGAGATTCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4926
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:95
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:TTAATTCCCTCGCACCCGAC
Suggested primer 2:AGAGGCTGCGTTTCTGGTAC