| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073489 |
| Chromosome: | chromosome 7 |
| Location: | 5954417 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354500 | HSF2 | (1 of 2) K09419 - heat shock transcription factor, other eukaryote (HSFF); Heat shock transcription factor 2 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAAACACCACAGCGGCCCCTTCGGAGCATCGGACCCACACACACACGC |
| Internal bar code: | GATAAGGGTAATGTTTCTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3259 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 106 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGCTGCGTTTCTGGTAC |
| Suggested primer 2: | TTAATTCCCTCGCACCCGAC |