Insertion junction: CLIP2.073492_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073492
Chromosome:chromosome 16
Location:4942857
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g686550 (1 of 1) PF12400 - Vaculolar membrane protein (DUF3661) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGCAGCGTGTGGCGCCGCACGCACCCTCCGCCTGTGCCCAATCCCATT
Internal bar code:TGGCTGACAAACTCTAAGGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:51
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGTGCTCTGCGCTTGTTC
Suggested primer 2:GCAACGAATGCGATACACCC