| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073495 |
| Chromosome: | chromosome 6 |
| Location: | 7237696 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298800 | ARS73A,ARS73a,LTH5 | Arylsulfatase 73a | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTATGTACATTGTTGTGGCTACTTACATCGGGATAGAGGCTCGAAACC |
| Internal bar code: | CCTCGTGTCGGAGGCATTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3261 |
| LEAP-Seq percent confirming: | 80.6452 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCATTCTTGCCATGCG |
| Suggested primer 2: | GAACGGCTCCAGAACCTGAA |