| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073496 |
| Chromosome: | chromosome 13 |
| Location: | 483389 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565050 | (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGCAAAACAAGCGCAATCGTCAGAATTCGATACCCTATACCGGCTTC |
| Internal bar code: | GATGGCAAATTCGTTGTGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGTCGGCGTGTGTGTAG |
| Suggested primer 2: | TGCACCTGATTCATTGGGCT |