| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.073497 |
| Chromosome: | chromosome 16 |
| Location: | 4713573 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677702 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGCAAGTATGACCACCAGCCCACGACGGGCGACTCTTGCCCTCCCCG |
| Internal bar code: | CAGTCACCAGTGGGCTTTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4503 |
| LEAP-Seq percent confirming: | 59.3496 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGGATGTAGTCGGGCTC |
| Suggested primer 2: | TCAATGCAACGGGCTAGGTT |