| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.073500 |
| Chromosome: | chromosome 3 |
| Location: | 4394974 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175000 | RPH3,RNPH3 | 3'-5' Exoribonuclease PH component of the Exosome; (1 of 1) PTHR11097:SF14 - EXOSOME COMPLEX COMPONENT RRP45 | 3'UTR |
| Cre03.g175050 | TRP13 | Transient receptor potential ion channel protein; (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATTCGTGCATTCACCGGCTGCCCTTTTGCGAGGCCAGGAGCACAATAG |
| Internal bar code: | GTCTCCATGGATATTATGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1528 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTGCTGGTCTGAGGATG |
| Suggested primer 2: | AAGAGGAGGAGGAGGAGCAG |