| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073513 |
| Chromosome: | chromosome 7 |
| Location: | 2900216 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCATGACTGGGCCCACGCCCACCACACACCCCGCCTGCACCTGCGC |
| Internal bar code: | AGGATTGGCTTAACACGTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 14.8148 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTGTTCGACGAAATGG |
| Suggested primer 2: | GGCAGTCCATGTTTCGCAAG |