| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073514 |
| Chromosome: | chromosome 15 |
| Location: | 961716 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639600 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCGCACCTCGGCCGGTTCCAAGGGCGCAGCTACTGCCGCTGCTACT |
| Internal bar code: | AACAAATTCTTCTTTGCTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTGCTACTGCCGCCAG |
| Suggested primer 2: | TAGACCTCCCTTCCCTCAGC |