| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073530 |
| Chromosome: | chromosome 2 |
| Location: | 652137 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077750 | FAP211,CSE12 | Flagellar Associated Protein 211 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCCTTGGAAGCCTGAGTGCCCGGCTAAGTGCGCCGCCTGCCCCTTCT |
| Internal bar code: | TTTTCGCTTAATTTAGGTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1158 |
| LEAP-Seq percent confirming: | 27.5 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACCTTGATGTTGATGCC |
| Suggested primer 2: | CACAGCTTGACAACCGTGTG |