Insertion junction: CLIP2.073531_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073531
Chromosome:chromosome 13
Location:5140286
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g607100 CYG6 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGATTTGATCGGTGTTTGCTCGCTGTTGGTCCTACCCGCTCTGCCCG
Internal bar code:TCACGCTACACGGTCCGGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:602
LEAP-Seq percent confirming:15.3846
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTGCTTCCAACTGCTGA
Suggested primer 2:GCACAATGCTCTGGTGTGTG