| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073553 |
| Chromosome: | chromosome 10 |
| Location: | 2723968 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437850 | SAE1,CBP20 | CAP-binding protein 20; (1 of 1) K12883 - nuclear cap-binding protein subunit 2 (NCBP2, CBP20) | 3'UTR |
| Cre10.g437900 | SPL31 | (1 of 1) PTHR17204//PTHR17204:SF29 - PRE-MRNA PROCESSING PROTEIN PRP39-RELATED // SUBFAMILY NOT NAMED; Pre-mRNA splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTTGACATGTTACCGCAGTTCGATGCCCATGAGTCCATCAGACGGTG |
| Internal bar code: | AAACGGCACAATCCTTTAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3132 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCAAGGAGGAGGTGAG |
| Suggested primer 2: | TGCGACCTTCATGCCTTCAT |