Insertion junction: CLIP2.073560_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.073560
Chromosome:plastome
Location:187617
Confidence (%):40
Locus systematic id Locus common name Defline Feature
CreCp.g802331 2716963,ChreCp066,psbC (1 of 1) K02705 - photosystem II CP43 chlorophyll apoprotein (psbC); photosystem II 43 kDa protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATAAATGTGCCAAATACCACCAAGGATTTCTAAAGTACCGATCCAAATG
Internal bar code:ATGCGCATACGTGATTTTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:239
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCGTGACCAAGAAACAAC
Suggested primer 2:TTTTCGAAACCAGCAGCAGC