Insertion junction: CLIP2.073575_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073575
Chromosome:plastome
Location:19582
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802271 ChreCp009,2717052,chlL light-independent protochlorophyllide reductase iron-sulfer ATP-binding protein; (1 of 1) K04037 - light-independent protochlorophyllide reductase subunit L (chlL) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTAAGAGGTGACATTCTTATATTTAATACATAATAGTTCAATTGTATA
Internal bar code:GATTGGGTCCGTCAGGGGCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGACGTTTGAAACCACC
Suggested primer 2:ACACCCCCATAACCTCCGTA