| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073578 |
| Chromosome: | chromosome 4 |
| Location: | 2774370 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCGGGGGGAACGGGGGCCGGGGGAGGTATGGGGTCGGACAGATAGAC |
| Internal bar code: | CGAGGTAATTAGTGGAAAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 16.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCTGGGGGTGATGGGAGG |
| Suggested primer 2: | AGCCAGAGTAAAAGCGTGCT |