| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073578 |
| Chromosome: | plastome |
| Location: | 75704 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802294 | ChreCp029,psbH,2717030 | photosystem II reaction center protein H; (1 of 1) K02709 - photosystem II PsbH protein (psbH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTGAGTTGCTTAGTTTACCTTAGCGAAAAGAAGACTTAGCAGCTAGC |
| Internal bar code: | GGCAGAATGGCGATTGATATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 4.7619 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAAAAGGGACTGCCTAC |
| Suggested primer 2: | GAATCCGCGTTTTCTCCGTG |