| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073580 |
| Chromosome: | chromosome 4 |
| Location: | 2364268 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221150 | GT90-5,GT90F5 | GT90 family protein 5; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGAAGGTCATTTCGGTGACTCAACCCGCAAGGCCATTACCTCCCCTG |
| Internal bar code: | CGATTCGAGAGGTTGCCGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1248 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTCCATTCAGCTTGACC |
| Suggested primer 2: | GCGGTGTGACTTTGTGTTCC |